research cell line source s wt hela  (ATCC)

Journal: Cancer Genomics & Proteomics

Article Title: NPM1 Drives ERK1/2-Dependent Tumor Progression in Lung Cancer

doi: 10.21873/cgp.20572

Figure Lengend Snippet: NPM1 expression is up-regulated in lung cancer tissues and cell lines. (A) Expression of NPM1 in lung cancer tissues and adjacent normal lung tissues based on TCGA RNA-seq datasets. NPM1 expression was significantly higher in lung cancer tissues compared to normal controls (***p<0.001). (B) Western blot analysis of NPM1 protein levels in a panel of lung cancer cell lines (A549, H460, Calu-1, H1299, H358, Calu-3, SK-MES-1) compared to the normal lung fibroblast cell line MRC-5. β-Actin was used as a loading control. All western blot experiments were independently repeated three times (biological replicates n=3).

Article Snippet: Human lung cancer cell lines (A549, H460, Calu-1, H1299, H358, Calu-3, SK-MES-1) and the normal lung fibroblast cell line (MRC-5) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, RNA Sequencing, Western Blot, Control

Journal: Microbial Cell Factories

Article Title: A novel green synthesized ZnO-based antimicrobial nanocomposite: synergistic action, in vitro cytotoxicity, and molecular docking studies of ceftazidime, metformin, and chitosan against multidrug-resistant Salmonella enterica

doi: 10.1186/s12934-026-02934-x

Figure Lengend Snippet: Dose-dependent cytotoxic effect of ZnO/CS/CAZ/MTF nanocomposite on WI-38 lung fibroblast cells determined via MTT assay

Article Snippet: The human lung fibroblast cell line (WI-38) was obtained from ATCC through the Holding company for biological products and vaccines (VACSERA) in Cairo, Egypt.

Techniques: MTT Assay

Journal: ACS Omega

Article Title: A Biocompatible and High-Sensitivity Lanthanide Coordination Complex for Luminescent Temperature Sensor Constructed from Non-Steroidal Anti-Inflammatory Drug Ketoprofen

doi: 10.1021/acsomega.5c11396

Figure Lengend Snippet: Cell viability of MRC-5 cells after 48 h of incubation with the mixed complex [Eu 0.4 Tb 1.6 (keto) 6 (bpy) 2 ], keto (ketoprofen), and bpy (2,2′-bipyridine) at concentrations ranging from 0.39 to 400 μM, measured by the MTT assay.

Article Snippet: The in vitro cytotoxicity assay of the heterometallic complex [Eu 0.4 Tb 0.6 (keto) 6 (bpy) 2 ], and its ligands ketoprofen (keto) and 2,2′-bipyridine (bpy) was assessed using the nontumoral human lung fibroblast cell line MRC-5 (ATCC CCL-171).

Techniques: Incubation, MTT Assay

Journal: Frontiers in Medical Technology

Article Title: Drug-loaded nanoparticles reduced platelet aggregation and blood coagulation

doi: 10.3389/fmedt.2025.1690389

Figure Lengend Snippet: Biocompatibility of anti-PLT LNPs. Light microscopy images of human fibroblasts cultured for 24 h with anti-PLT LNPs mixed with a growth medium at ratios of 0:1 (control), 0.5:1 (9.60 ± 0.09 × 10 −3 mol NO exposure), and 1:1 (19.20 ± 3.68 × 10 −3 mol NO exposure) show that cell population density in the 0.5:1 group was comparable to that in the control group. In contrast, a reduction in cell density was observed at the 1:1 ratio, consistent with the known antiproliferative effects of higher NO concentrations.

Article Snippet: The WI-38 human lung fibroblast cell line was purchased from ATCC (Manassas, VA, USA).

Techniques: Light Microscopy, Cell Culture, Control